References

AIM OA Sys – veterinary monitoring. 2024. https://www.aim-oa.com/

Armitage AJ, Miller JM, Sparks TH, Georgiou AE, Reid J Efficacy of autologous mesenchymal stromal cell treatment for chronic degenerative musculoskeletal conditions in dogs: a retrospective study. Front Vet Sci.. 2023; 9 https://doi.org/10.3389/fvets.2022.1014687

Blázquez R, Sánchez-Margallo FM, Reinecke J Conditioned serum enhances the chondrogenic and immunomodulatory behavior of mesenchymal stem cells. Front Pharmacol.. 2019; 10 https://doi.org/10.3389/fphar.2019.00699

Brown DC The canine orthopedic index. Step 1: devising the items. Vet Surg.. 2014; 43:(3)232-240 https://doi.org/10.1111/j.1532-950X.2014.12142.x

Brown DC, Boston RC, Coyne JC, Farrar JT Ability of the canine brief pain inventory to detect response to treatment in dogs with osteoarthritis. J Am Vet Med Assoc.. 2008; 233:(8)1278-1283 https://doi.org/10.2460/javma.233.8.1278

Brown DC, Boston RC, Farrar JT Comparison of force plate gait analysis and owner assessment of pain using the Canine Brief Pain Inventory in dogs with osteoarthritis. J Vet Intern Med.. 2013; 27:(1)22-30 https://doi.org/10.1111/jvim.12004

Budsberg SC, Verstraete MC, Soutas-Little RW Force plate analysis of the walking gait in healthy dogs. Am J Vet Res.. 1987; 48:(6)915-918

Canapp SO, Canapp DA, Ibrahim V, Carr BJ, Cox C, Barrett JG The use of adipose-derived progenitor cells and platelet-rich plasma combination for the treatment of supraspinatus tendinopathy in 55 dogs: a retrospective study. Front Vet Sci.. 2016a; 3 https://doi.org/10.3389/fvets.2016.00061

Canapp SO, Leasure CS, Cox C, Ibrahim V, Carr BJ Partial cranial cruciate ligament tears treated with stem cell and platelet-rich plasma combination therapy in 36 dogs: a retrospective study. Front Vet Sci.. 2016b; 3 https://doi.org/10.3389/fvets.2016.00112

Canine Arthritis Management. Your dog, arthritis and how CAM can help. 2024. https://caninearthritis.co.uk

Carr BJ, Canapp SO, Mason DR, Cox C, Hess T Canine platelet-rich plasma systems: a prospective analysis. Front Vet Sci.. 2016; 2 https://doi.org/10.3389/fvets.2015.00073

Clarke E, Aulakh KS, Hudson C Effect of sedation or general anesthesia on elbow goniometry and thoracic limb circumference measurements in dogs with naturally occurring elbow osteoarthritis. Vet Surg.. 2020; 49:(7)1428-1436 https://doi.org/10.1111/vsu.13499

Clough WT, Canapp SO, Taboada L, Dycus DL, Leasure CS Sensitivity and specificity of a weight distribution platform for the detection of objective lameness and orthopaedic disease. Vet Comp Orthop Traumatol.. 2018; 31:(6)391-395 https://doi.org/10.1055/s-0038-1667063

Davies V, Reid J, Wiseman-Orr ML, Scott EM Optimising outputs from a validated online instrument to measure health-related quality of life (HRQL) in dogs. PLoS One.. 2019; 14:(9) https://doi.org/10.1371/journal.pone.0221869

DeLuke AM, Allen DA, Wilson ER Comparison of radiographic osteoarthritis scores in dogs less than 24 months or greater than 24 months following tibial plateau leveling osteotomy. Can Vet J.. 2012; 53:(10)1095-1099

Fahie MA, Ortolano GA, Guercio V A randomized controlled trial of the efficacy of autologous platelet therapy for the treatment of osteoarthritis in dogs. J Am Vet Med Assoc.. 2013; 243:(9)1291-1297 https://doi.org/10.2460/javma.243.9.1291

Formenton MR, de Lima LG, Vassalo FG, Joaquim JGF, Rosseto LP, Fantoni DT Goniometric assessment in French Bulldogs. Front Vet Sci.. 2019; 6 https://doi.org/10.3389/fvets.2019.00424

Fox EA, Oliver KE, Brunke MW Evaluation of a method to measure the ratio of pelvic limb to thoracic limb girth in dogs. Vet Evid.. 2023; 8:(4)1-9 https://doi.org/10.18849/ve.v8i4.667

García-Bernal D, García-Arranz M, Yáñez RM The current status of mesenchymal stromal cells: controversies, unresolved issues and some promising solutions to improve their therapeutic efficacy. Front Cell Dev Biol.. 2021; 9 https://doi.org/10.3389/fcell.2021.650664

Hielm-Björkman AK, Rita H, Tulamo RM Psychometric testing of the Helsinki chronic pain index by completion of a questionnaire in Finnish by owners of dogs with chronic signs of pain caused by osteoarthritis. Am J Vet Res.. 2009; 70:(6)727-734 https://doi.org/10.2460/ajvr.70.6.727

Hsu WK, Mishra A, Rodeo SR Platelet-rich plasma in orthopaedic applications: evidence-based recommendations for treatment. J Am Acad Orthop Surg. 2013; 21:(12)739-748 https://doi.org/10.5435/JAAOS-21-12-739

Hudson JT, Slater MR, Taylor L, Scott HM, Kerwin SC Assessing repeatability and validity of a visual analogue scale questionnaire for use in assessing pain and lameness in dogs. Am J Vet Res.. 2004; 65:(12)1634-1643 https://doi.org/10.2460/ajvr.2004.65.1634

Jaegger G, Marcellin-Little DJ, Levine D Reliability of goniometry in Labrador Retrievers. Am J Vet Res. 2002; 63:(7)979-986 https://doi.org/10.2460/ajvr.2002.63.979

Kujala MV, Cardó AV, Somppi S Comparison of activity trackers in estimating canine behaviors. Adv Robot.. 2024; 38:(14)934-946 https://doi.org/10.1080/01691864.2024.2343080

Linon E, Spreng D, Rytz U, Forterre S Engraftment of autologous bone marrow cells into the injured cranial cruciate ligament in dogs. Vet J.. 2014; 202:(3)448-454 https://doi.org/10.1016/j.tvjl.2014.08.031

McLellan J, Plevin S Does it matter which platelet-rich plasma we use?. Equine Vet Educ.. 2011; 23:(2)101-104 https://doi.org/10.1111/j.2042-3292.2010.00185.x

Miller J, Standen C, Frost L Effect of a dog's age on stem cell culture times and efficacy outcomes in dogs with osteoarthritis treated with intraarticular injections of autologous mesenchymal stem cells.Gloucester: BSAVA; 2018

Mohoric L, Zorko B, Ceh K, Majdic G Blinded placebo study of bilateral osteoarthritis treatment using adipose derived mesenchymal stem cells. Slov Vet Res.. 2016; 53:(3)167-174

Mölsä SH, Hyytiäinen HK, Hielm-Björkman AK, Laitinen-Vapaavuori OM Long-term functional outcome after surgical repair of cranial cruciate ligament disease in dogs. BMC Vet Res.. 2014; 10 https://doi.org/10.1186/s12917-014-0266-8

Moreau M, Lussier B, Ballaz L, Troncy E Kinetic measurements of gait for osteoarthritis research in dogs and cats. Can Vet J.. 2014; 55:(11)1057-1065

Explanation of grading according to IEWG and discussion of cases. 2015. https://www.vin.com/apputil/content/defaultadv1.aspx?pId=14365&catId=73679&id=7259256

Pelletier MH, Malhotra A, Brighton T, Walsh WR, Lindeman R Platelet function and constituents of platelet rich plasma. Int J Sports Med.. 2013; 34:(1)74-80 https://doi.org/10.1055/s-0032-1316319

Reid J Measuring quality of life in dogs and cats with osteoarthritis. Companion Animal.. 2024; 29:(Suppl 9)17-23 https://doi.org/10.12968/coan.2024.29.Sup9.17

Reid J, Wiseman-Orr ML, Scott EM, Nolan AM Development, validation and reliability of a web-based questionnaire to measure health-related quality of life in dogs. J Small Anim Pract.. 2013; 54:(5)227-233 https://doi.org/10.1111/jsap.12059

Sabanci SS, Ocal MK Comparison of goniometric measurements of the stifle joint in seven breeds of normal dogs. Vet Comp Orthop Traumatol.. 2016; 29:(3)214-219 https://doi.org/10.3415/VCOT-15-05-0090

Skangals U Stem cell therapy in the treatment of elbow joint osteoarthritis in dogs. E J Vet Med.. 2022; 2:(3)1-8 https://doi.org/10.24018/ejvetmed.2022.2.3.30

Thomovsky SA, Chen AV, Kiszonas AM, Lutskas LA Goniometry and limb girth in miniature Dachshunds. J Vet Med.. 2016; 2016 https://doi.org/10.1155/2016/5846052

Upchurch DA, Renberg WC, Roush JK, Milliken GA, Weiss ML Effects of administration of adipose-derived stromal vascular fraction and plateletrich plasma to dogs with osteoarthritis of the hip joints. Am J Vet Res.. 2016; 77:(9)940-951 https://doi.org/10.2460/ajvr.77.9.940

Vilar JM, Morales M, Santana A Controlled, blinded force platform analysis of the effect of intraarticular injection of autologous adipose-derived mesenchymal stem cells associated to PRGFEndoret in osteoarthritic dogs. BMC Vet Res.. 2013; 9 https://doi.org/10.1186/1746-6148-9-131

Walton MB, Cowderoy E, Lascelles D, Innes JF Evaluation of construct and criterion validity for the ‘Liverpool Osteoarthritis in Dogs’ (LOAD) clinical metrology instrument and comparison to two other instruments. PLoS One.. 2013; 8:(3) https://doi.org/10.1371/journal.pone.0058125

Wiseman-Orr ML, Scott EM, Reid J, Nolan AM Validation of a structured questionnaire as an instrument to measure chronic pain in dogs on the basis of effects on health-related quality of life. Am J Vet Res.. 2006; 67:(11)1826-1836 https://doi.org/10.2460/ajvr.67.11.1826

Wits MI, Tobin GC, Silveira MD Combining canine mesenchymal stromal cells and hyaluronic acid for cartilage repair. Genet Mol Biol.. 2020; 43:(1) https://doi.org/10.1590/1678-4685-GMB-2019-0275

Yoon H-Y, Lee J-H, Jeong S-W Long-term follow-up after implantation of autologous adipose tissue derived mesenchymal stem cells to treat a dog with stifle joint osteoarthrosis. J Vet Clin.. 2012; 29:(1)82-86

Yun S, Ku SK, Kwon YS Adipose-derived mesenchymal stem cells and platelet-rich plasma synergistically ameliorate the surgical-induced osteoarthritis in Beagle dogs. J Orthop Surg Res.. 2016; 11 https://doi.org/10.1186/s13018-016-0342-9

Stem cells in canine osteoarthritis. Part 2: case selection, choices, guidelines and outcomes

02 April 2025
14 mins read
Volume 30 · Issue 4

Abstract

This article, part 2 of a 3-part series covering the use of stem cells in canine osteoarthritis, discusses case selection, intra-articular therapies, guidelines for joint injections and clinical metrology in the use of regenerative therapy techniques for osteoarthritis in dogs. Osteoarthritis is a prevalent condition in dogs, and can have a negative effect on quality of life. It is a chronic condition which requires long-term management, and stem cells are a potential method of improving quality of life in affected animals.

Osteoarthritis is frequently seen in veterinary clinics, affecting up to 20% of dogs in the UK (based on referral data). In order to reduce the negative effects that this chronic condition can have on affected dogs, research into using stem cells to benefit these animals is ongoing. The various ways that stem cell therapy can be applied to help these dogs are discussed in this article, which is part 2 of a 3-part series around the use of stem cells in canine osteoarthritis.

In practice, the most common uses of stem cell therapy in dogs are:

Appropriate diagnostic techniques should be performed, and may include:

Training in the following modalities would be recommended:

Patients are selected according to several criteria, and different clinicians will prefer one selection process over another. A ‘candidacy check’ process to determine suitability for treatment and maximise the potential for a successful outcome includes the study of previous clinical history, a specific series of owner-submitted videos of the patient and an in-depth consultation, as well as owner-derived osteoarthritis and quality of life scores.

Register now to continue reading

Thank you for visiting UK-VET Companion Animal and reading some of our peer-reviewed content for veterinary professionals. To continue reading this article, please register today.