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Essential haematology for companion animal vets in first opinion practice

02 June 2023
11 mins read
Volume 28 · Issue 6
Figure 3. a) Stained blood smear before application of oil. b) Slide after application of oil.
Figure 3. a) Stained blood smear before application of oil. b) Slide after application of oil.

Abstract

Haematology provides a vital source of diagnostic information in veterinary medicine. Advancement in technology has improved the availability of in-house automated analysers in first opinion practices, allowing practitioners quick access to complete blood count results to aid day-to-day clinical decisions. Most in-house analysers use flow cytometry or electrical impedence technologies which provide fairly accurate complete blood count results. However, practitioners must exercise care when interpreting these results as sample factors such as lipaemia, haemolysis, and the presence of atypical white blood cells, may have an effect on the accuracy of the results. Interpreting complete blood count results alongside a good quality blood smear is important, as it serves as a quality control tool to assess the accuracy of the complete blood count results and also provides useful information on the cell morphology. Good sample handling and good smear making technique are important to produce a blood smear of diagnostic value.

Haematology is an integral part of veterinary medicine, providing useful baseline information in situations such as routine screening tests or pre-anaesthetic blood tests, as well as diagnostic purposes. In-house haematology analysers are becoming more readily available for first opinion practices, allowing practitioners the benefit of rapid complete blood count results, which is particularly useful in cases of critical illness or other time-sensitive situations. However, practitioners must exercise care when interpreting these complete blood count results, because of there is a possibility of unreliable results arising from sample and patient factors, or from analyser factors, as in-house analysers can lack the vigorous quality control procedures that are performed by reference laboratories. A good quality blood smear examined alongside analyser results can help assess analyser accuracy and provide important information about cell morphology. Good sample handling and smear-making technique are important, as is a good understanding of smear interpretation. This article describes the important features of automated analysers, alongside the preparation and use of a good quality blood smear.

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